Figure 4.
Studies of XIAP inhibitor 1396-12 in combination with Ara-C. (A) OCI AML2 and Jurkat leukemia cells (6.25 × 105/mL) were treated with increasing concentrations of 1396-12 with (▴) or without (▪) Ara-C. Twelve hours after incubation, apoptosis was measured by annexin V staining. (B) HL60 leukemia cells (6.25 × 105/mL) or primary AML patient samples (AML Pt 1-3) (× 105/mL) were treated with increasing concentrations of Ara-C alone (▪) or in combination with 8 μM (HL60) or 10 μM (AML patient samples) of the active XIAP inhibitor 1396-12 (▴) or the inactive control 1396-28 (•). Apoptosis was measured by annexin V staining 48 hours after incubation. The mean percentage plus or minus SD of viable cells is shown.
