Viral vectors activate dsRNA-triggered antiviral and PI3 kinase/Akt pathways. Following viral infection (MOI 500), iDCs (left column) and mDCs (right column; activated with 20 ng/mL TNF-α, 20 ng/mL IL-1β, 20 ng/mL LPS, and 10 ng/mL PGE₂ for 48 hours) were analyzed. Five days after transduction, the DCs were analyzed by Western blotting for expression of phosphorylated PKR, phosphorylated EIF-2α, phosphorylated IκBα, and phosphorylated PI3 kinase as well as β-actin. In addition, RT-PCR was used to analyze mRNA levels of 2′-5′-OAS, IRF-1, IFN-α and -β, and β-actin.