Change in phenotype of iDCs following viral transduction. Following transduction of iDCs with Ad0 (third column; MOI 500), AdGFP (fourth column; MOI 500), HIV-GFP (fifth column; MOI 500), or EIAV-GFP (sixth column; MOI 500), the phenotype of the cells was analyzed using 2-color flow cytometry for the expression of (e)GFP (x axis) and the surface marker indicated (y axis). As a control, untransfected cells were either unstimulated (iDCs, first column) or activated with 20 ng/mL TNF-α, 20 ng/mL IL-1β, 20 ng/mL LPS, and 10 ng/mL PGE₂ for 48 hours (mDCs, second column). The results shown are representative of 3 experiments. The percentage of cells in each quadrant of the flow cytometry profiles is shown in the diagram beneath each profile.