Analysis of primary erythroleukemic cell growth cocultured with LDSACs, HDSACs, or their conditioned media. (A) Primary erythroleukemic (suspension) cells, seeded at a density of 5 × 104/mL, were cocultured with adherent cells derived in vitro either from healthy controls or mice infected with F-MuLV and presenting with Friend disease. Scoring of live cells occurred every 24 hours by trypan blue exclusion over a 72-hour time course with significance considered between LDSAC and HDSAC cocultures to reflect P < .05. (B) Primary erythroleukemic cells, seeded at a density of 1 × 104/mL, were cultured in conditioned medium previously used to grow LDSACs (○) versus HDSACs (□), hence LDSAC- and HDSAC-derived media, respectively. Their respective controls (•, ▪) represent similar experiments conducted in control media. Scoring of live cells occurred every 24 hours by trypan blue exclusion over a 72-hour time course with significance considered between different growth conditions to reflect P < .05 (*). Error bars indicate ± standard deviation.
