SDS-PAGE analysis of purified ANV and its fusion products with various Kunitz-type inhibitors. Samples were analyzed by 12% SDS-PAGE followed by Coomassie blue staining. All samples were boiled for 3 minutes without (A) or with (B) 50 mM dithiothreitol. Approximately 5 μg protein was loaded on each lane. (A-B) Lane 1, molecular weight (MW) markers; lane 2, ANV-KK_TFPI; lane 3, ANV-6L15; lane 4, TAP-ANV; lane 5 ANV-K_APP; lane 6, ANV.