Ex vivo–expanded BM HSCs are CD62L^-, IGF2-hFc⁺, CD31⁺, Tie-2^-, and prominin-1^-. After 10 days of culture of total BM cells, 4 × 10⁴ sorted CD62L⁺ and CD62L^- cells, or IGF2-hFc⁺ and IGF2-hFc^- cells, or Mpl⁺ and Mpl^- cells, or CD31⁺ and CD31^- cells, or 6000 sorted Tie-2⁺ and Tie-2^- cells, or 6000 prominin-1⁺ and prominin-1^- cells were transplanted, respectively, together with 10⁵ CD45.1 competitor cells into lethally irradiated CD45.1 mice (n = 4). Peripheral blood engraftments at 4 months after transplantation are shown. Error bars indicate SEM.