Flow cytometric analyses of monocytes and platelets from whole blood. Monocytes (A) and platelets (B) in unstimulated blood. Monocytes (C) and platelets (D) in LPS-stimulated blood. α–TF-5 was used for immunostaining. The positive analysis regions (vertical bars) were defined such that less than 99% of the cells stained with an irrelevant, isotype-matched mouse IgG were positive. The percentages shown are the percent of positive cells in these analysis regions.