Thrombin generation in synthetic plasma. Synthetic plasma was composed of factors V, VIII, VII, VIIa, IX, X, and XI, prothrombin, TFPI, and AT-III at their mean physiologic concentration. (A) Thrombin generation was initiated with 2 × 10⁸/mL A23187-treated platelets in the absence of TF and either in the presence (▴) or in the absence (□) of factors VII and VIIa. (B) Thrombin generation was initiated either with 2 × 10⁸/mL A23187-treated platelets in the absence (▴) or presence of 0.1 mg/mL α–TF-5 (▪) or with TF in the presence of 2 × 10⁸/mL resting platelets and either in the absence (▵) or presence of α–TF-5 (□). ○ represents thrombin generation in the presence of resting platelets and absence of TF.