Effect of a CD49d antagonist on neutrophil mobilization stimulated by MIP-2. Using the in situ perfusion system of the rat femoral bone marrow, the CD49d antagonist CT5627 (200 nM) or vehicle was infused for a total of 80 minutes, and MIP-2 (1 nM) or vehicle (PBS/0/1% BSA) was infused for 10 minutes, as indicated by the solid bars. (Left) The kinetics of neutrophil mobilization from the perfused femoral bone marrow (•, MIP-2 alone; ▴, MIP-2 + CT5627; ○, vehicle; ▵, vehicle + CT5627) and (right) the total number of neutrophils mobilized are shown on the right-hand axis, mean ± SEM (n = 5 separate perfusions). ^*P < .05 for both panels.