Accelerated myeloid reconstitution on transplantation of LSKCD34⁺flt3^– ST-HSCs. (A) Number of granulocytes (left) and macrophages (right) generated in vitro per initiating LSKCD34^–flt3^– (▴), LSKCD34⁺flt3^– (○), or LSKCD34⁺flt3⁺ (•) cell over time. Data are from 3 experiments. (B) In vivo competitive reconstituting ability of LSKCD34^–flt3^– LT-HSCs and LSKCD34⁺flt3^– ST-HSCs at day 8 (left) and 11 (right) following transplantation of 500 cells, in competition with 1 × 105 total BM cells. Data are from 8 to 14 mice from 2 experiments, as indicated in parentheses. Horizontal bars indicate mean values. *P < .05.