Effect of IMD-0354 on SCF-dependent proliferation of CBhCMCs. (A) CBhCMCs (105 cells/mL) were incubated in the presence of increasing concentrations of IMD-0354 (□) or STI571 (▪) for 48 hours with 100 ng/mL SCF, and proliferative activity of cells was evaluated through MTT assay. Each value represents the mean ± SE of 6 different experiments. (B) After incubation with various concentrations of IMD-0354, nuclear extracts were collected from CBhCMCs. NF-κB activity in nuclear extracts was analyzed with the use of a chemiluminescence transcription factor assay. Positive reactions were detected using a luminometer. None indicates medium alone; Consensus, consensus oligonucleotides; Mutant, mutant oligonucleotides. Each value represents the mean ± SE of 3 different experiments.
