Histochemical and immunohistochemical analysis of murine cardiac tissue after LPS administration. H&E staining of WT (A) and EPCRδ/δ (D) mouse heart sections 6 hours after LPS administration. (B,E) Anti-CD45 immunostaining of serial heart sections of WT and EPCRδ/δ mice, respectively, 6 hours after LPS administration. (C,F) Antineutrophil protein immunostaining of serial heart sections of WT and EPCRδ/δ mice, respectively, 6 hours after LPS administration. (G-L) Similar H&E (G,J), anti-CD45 (H,K), and antineutrophil protein (I,L) staining and immunostaining of WT and EPCRδ/δ mice, respectively, at 24 hours after LPS treatment. A Nikon Eclipse E600 light microscope (Nikon, Melville, NY) with a Nikon Plan Fluor objective (magnification, × 20; numerical aperture, 0.5). Images were captured with a SPOT 2 slider (model 1.4.0) camera (Diagnostic Instruments, Sterling Heights, MI) using SPOT software. The images were transferred to Adobe Photoshop 7.0 (Adobe, San Jose, CA) for final reproduction.
