Construction of the vectors pPCYTO/CHEM and pPC/HYPO. The basic vectors contain the sequences for the PCR fragments of RPL19 and HPRT between T7 and T3 RNA polymerase recognition sites that facilitate in vitro RNA synthesis bi-directionally. (A) There is a multiple cloning site (MCS) between the sequences of the housekeeping genes RPL19 and HPRT. The MCS contains several restriction enzyme recognition sites. (B) The pCYTO/CHEM plasmid contains the sequences for the PCR fragments of TNFα, IL1β, IL6, MCP-1, and MIP-2. (C) The pPC/HYPO plasmid contains the sequences for the PCR fragments of TM, EPCR, PC, iNOS, HIF1α, and VEGF.