Ab reactivity with cultured bone marrow cells and freshly isolated spleen cells. (A) The 2E6⁺ cells are compatible with pDCs. Bone marrow cells from BALB/c mice were cultured in the presence of Flt-3L for 10 days to generate pDCs in vitro. Cells were then stained with 2E6, CD11c, CD11b, B220, and Ly6C. Profiles showed 2E6⁺ gate (R1). (B) The 2E6 does not express on myeloid DCs. Bone marrow cells from BALB/c mice were cultured in the presence of GM-CSF for 10 days and then stained with CD11b, CD11c, and 2E6 Ab. At day 10, the majority of the cells (58%) became CD11b⁺CD11c⁺ myeloid DCs. Percentages in quadrants indicate the percentage of positive cells in that quadrant.