Expression of Abcg2 in MEL cells before and after induction with DMSO and in primary erythroblasts during differentiation. (A) Noninduced MEL cells and MEL cells induced with 2% DMSO for 4 days were analyzed for Abcg2 mRNA by Northern blot, glyceraldehyde phosphate dehydrogenase (GAPDH)probe served as loading control. (B) Flow cytometry analysis of MEL cells after staining with anti-Abcg2 antibody Bxp-53. (C) Hoechst 33342 fluorescence in cells analyzed by flow cytometry. Shaded area in B and C indicates noninduced MEL cells; solid line in B and C, MEL cells induced with DMSO. (D) Murine splenic proerythroblasts were cultured for differentiation; samples were taken at different time points and measured for Abcg2 mRNA using microarray. Baso indicates basophilic erythroblasts; poly, polychromatic erythroblasts; and ortho, orthochromatic erythroblasts.