Activity of RAPA on various AML cell lines. KG1, KG1a, U937, K562, UT-7EPO, HEL, HL60, and UT-7GM AML cell lines were grown in clonogenic assays in the presence of increasing doses of RAPA (▪, 0 nM; ▦, 1 nM; ▧, 10 nM; □, 100 nM). Results are presented as percentage of CFU-L for each RAPA-treated AML cell line relative to untreated cells and are mean ± SEM of 3 independent experiments performed in duplicate.