American Society of Hematology

Figure 7.

$Figure 7. Identification of the subpopulation of progenitor cells from untreated spleen that responds to BMP4. (A) Unfractionated, Lin+ and Lin– cells from untreated wild-type spleen were plated in 3 U/mL Epo without (▪) or with 15 ng/mL BMP4 (▦) and the induction of stress BFU-Es was analyzed. (B) Kit+Lin– and Kit–Lin– cells from untreated wild-type spleen were plated in 3 U/mL Epo without (▪) or with 15 ng/mL BMP4 (▦) and the induction of stress BFU-Es was analyzed. (C) MEPs (Lin–Sca1-IL-7Rα–Kit+CD34-FcγRlow) isolated from bone marrow and spleen of wild-type mice were plated in 3 U/mL Epo without (▪) or with 15 ng/mL BMP4 (▦) and the induction of stress BFU-Es was analyzed. N.D. indicates none detected. Error bars represent SEM.$

Identification of the subpopulation of progenitor cells from untreated spleen that responds to BMP4. (A) Unfractionated, Lin⁺ and Lin^– cells from untreated wild-type spleen were plated in 3 U/mL Epo without (▪) or with 15 ng/mL BMP4 (▦) and the induction of stress BFU-Es was analyzed. (B) Kit⁺Lin^– and Kit^–Lin^– cells from untreated wild-type spleen were plated in 3 U/mL Epo without (▪) or with 15 ng/mL BMP4 (▦) and the induction of stress BFU-Es was analyzed. (C) MEPs (Lin^–Sca1-IL-7Rα^–Kit⁺CD34-FcγR^low) isolated from bone marrow and spleen of wild-type mice were plated in 3 U/mL Epo without (▪) or with 15 ng/mL BMP4 (▦) and the induction of stress BFU-Es was analyzed. N.D. indicates none detected. Error bars represent SEM.

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