Analysis of the recovery from acute anemia in f/Madh5^– and+/Madh5^– mice. (A) Analysis of the expansion of stress BFU-Es in the spleen of C57BL/6-f/Madh5^– (□) and C57BL/6-+/Madh5^– mice (▪). Spleen cells were plated in methylcellulose media containing 3 U/mL Epo. (B) Expression of BMP4 in the spleen of C57BL/6-f/Madh5^– and C57BL/6-+/Madh5^– mice. The BMP4 and HPRT control bands are indicated by the arrows. These results are from 25 cycles of PCR. Error bars represent SEM.