Phenotypic characteristics of monocyte-derived mature DCs. mDCs were generated from blood monocytes as described. Nonadherent cells were stained with fluorochrome-conjugated mouse antihuman monoclonal antibodies. FACScan data show the cell surface expression of the indicated marker (gray area) or isotope controls (bold lines) with a life gate from one representative experiment of DCs matured with CD40L + LPS.