Dose-dependent vaccine effect, and induction of memory immunity, by vaccination with irradiated DA1-3b leukemic cells transduced with the gene for CXCL10. (A) C3H/Hej mice were vaccinated with 106 irradiated DA1-3b/CXCL10 cells and then challenged by 104 wild-type DA1-3b cells (•). Mice that survived this were rechallenged 3 months later with the same dose of leukemic cells. A control group of 10 naive mice was vaccinated with DA1-3b/Zeo cells and challenged at the same time (▪). (B) Same procedure as in panel A but mice were rechallenged with 105 wild-type DA1-3b cells. (C) C3H/Hej mice were vaccinated with 105 (○), 106 (•), or 107 irradiated DA1-3b/CXCL10 cells (
), and the mice were subsequently challenged with 105 wild-type DA1-3b cells. ▪ represents control cells.
