Irradiated DA1-3b/CXCL10 leukemic cells induce prophylactic and therapeutic immunity. (A-C) Vaccine experiments. C3H/Hej mice were vaccinated with 10⁶ irradiated DA1-3b leukemic cells that had been transduced with CXCL10 (DA1-3b/CXCL10; •), IL-12 (DA1-3b/IL-12; ▿), or control empty plasmid (DA1-3b/Zeo; ▪), and the mice were subsequently challenged with (A) 10⁴, (B) 10⁵, or (C) 10⁶ wild-type DA1-3b cells. (D-F) Therapeutic experiments. C3H/Hej mice were injected with (D) 10⁴, (E) 10⁵,or (F) 10⁶ wild-type DA1-3b/cells. Three days later mice were given injections of irradiated DA1-3b/CXCL10, DA1-3b/IL12, or DA1-3b/Zeo leukemic cells. Data represent 30 mice/group in 3 separate experiments.