Effects of dexamethasone on expression of Bcl-2 family members and PARP cleavage in xenografts ALL-3, ALL-7, ALL-16, and ALL-19. Xenograft cells were exposed to dexamethasone (1 μM) as indicated in the legend to Figure 5. (A) Total cellular protein (50 μg) was separated by SDS-PAGE and immunoblotted for actin, Bim, Bcl-2, Bax, and PARP. C indicates solvent-treated controls. (B) Quantified expression of the 3 major isoforms of Bim normalized as a percentage of actin controls. Symbols indicate same information as in Figure 5D. Data presented are the mean ± SEM of 3 independent experiments.