C1INH protects C57BL/6J mice from increased cutaneous vascular permeability in response to local LPS injection. (A) C57BL/6J mice were given subcutaneous injections of LPS at the indicated doses in the abdominal skin (n = 9 with the 10 μg dose, 9 with 25 μg, and 8 with 50 μg). Control mice were injected with PBS alone (n = 7). (B) Mice were injected subcutaneously in the abdominal skin with LPS (10 μg) alone (n = 8), a mixture of LPS (10 μg) with C1INH (200 μg; n = 8) (SC), or LPS (10 μg) subcutaneously with an intravenous dose of C1INH (200 μg; n = 8)(IV). Control mice were injected with PBS (n = 7) or with C1INH (n = 7) alone. Experimental data were presented as means ± standard deviation using GraphPad Prism 3.0 software (GraphPad Software, San Diego, CA).