The effect of C1INH on LPS-induced endothelial cell detachment in vitro. The cell line HUV-EC-C (A) and primary HUVECs (B) were incubated with LPS (175 ng/mL) in the absence (▿) or presence of C1INH (150 μg/mL; □). Control cultures were treated with PBS alone (○). The cells in the supernatant and wash, as well as those detached from the filter with trypsin were stained with 0.4% Trypan Blue and counted. The percentage of cell detachment was calculated as described in “Materials and methods.”