Plasmid vectors. pSBT/mCAGF8 contains a cytomegalovirus (CMV) enhancer/beta-actin chimeric promoter with a mini-intron (mini-Caggs; see Materials and methods) regulating expression of a BDD hFVIII cDNA followed by a rabbit beta-globin polyadenylation signal. The inverted repeat (IR) sequences serve as transposase binding sites and thus form the termini of the integrating unit, which is roughly 6.4 kb in length. pUB-SB10 contains a first generation “SB-10” transposase sequence regulated by a ubiquitin C promoter followed by an SV40 polyadenylation signal. pGFP is regulated by a CMV promoter, and pCAGGS-LUC is regulated by a full-length CAG promoter, each containing a rabbit beta-globin polyadenylation signal.