Binding of ICAM-1 to DBLβC2 chimeric and deletion constructs. Chimeric and deletion constructs based on the ICAM-1–binding DBLβC2 domain derived from JDP8Icvar (JDP8-DBLβC2) and DBLβC2 domain derived from FCR3varCSA (FCR3-DBLβC2), which does not bind ICAM-1, were expressed on surface of COS-7 cells and tested for binding to ICAM-1. Transfection efficiency for each construct is shown. Number of transfected COS-7 cells that bind ICAM1-Fc–coated magnetic beads scored in 25 fields at × 200 magnification and normalized to transfection efficiency of 20% is shown. No binding was seen in the entire well where zero binding is reported. Positions of cysteines are shown with sticks. ND indicates not done.