Induction of IL-8 gene expression and secretion in HMECs. Quantitative RT-PCR of IL-8 (A) and ICAM-1 (C) determined their amount of transcription in MRP8/MRP14-treated (▪) and control HMECs (▦). Columns represent mean ± SD of 4 independent experiments (^*P < .001). (B) The concentration of IL-8 was determined in supernatants of MRP8/MRP14-treated and control HMEC monolayers and set in relation to LDH activity to exclude differences caused by cell death. Columns represent mean ± SD of 3 independent experiments (^*P < .05). (D) MRP8/MRP14-treated and control HMECs were immunostained for ICAM-1 or incubated with IgG isotype control mAb. ICAM-1 expression was analyzed by flow cytometry.