Primary DC types differ in their antigen-presenting capacities. (A) FACS analysis of CD1c⁺ PBDCs, plasmacytoid DCs, and B cells directly after isolation from a healthy donor. Primary antigen-presenting cells were pulsed with NY-ESO-1 protein, NY-ESO-1/IC, or NY-ESO-1/IMX at the indicated concentrations, cultured overnight in medium containing GM-CSF for CD1c⁺ PBDCs or IL-3 for plasmacytoid DCs, and cocultured with NY-ESO-1–specific T cells. IFN-γ production by CD8⁺ T cells (B) and CD4⁺ T cells (C) was quantified by ICS. indicates NY-ESO-1; □, NY-ESO-1/IC; •, NY-ESO-1/IMX. Data are representative of 5 experiments.