Bcl-2 overexpression in the 697 cell line alters the pathway of apoptosis used. (A) Bcl-2 overexpression alters pathway of apoptosis used. Protein lysates were collected from 697-Neo and 697–Bcl-2 cells lines after incubation with media (M), 1 μM, or 10 μM OSU0312 and 25 μM 2-F-ara-A for 24 hours. Lysates were probed for PARP and GAPDH by immunoblot. Data are representative of 2 experiments. (B) Bcl-2 overexpression alters pathway of apoptosis used. Protein lysates were collected from 697-Neo and 697–Bcl-2 cells lines after incubation with media (M), 1 μM, or 10 μM OSU03012 and 25 μM 2-F-ara-A for 24 hours. Lysates were probed for caspase-3 and GAPDH by immunoblot. Data are representative of 2 experiments.