Bcl-2 overexpression in the 697 cell lines does not protect against OSU03012-mediated cytotoxicity. (A) Bcl-2 expression does not protect against OSU03012-mediated cytotoxicity. To show Bcl-2 overexpression in 697 Bcl-2 stably transfected cell line increasing amounts of protein cell lysates from 697-Neo and 697–Bcl-2 cells lines were probed for Bcl-2 and GAPDH (loading control). (B) 697-Neo (▪) and 697 cell lines (▦) were incubated with increasing doses of OSU03012 for 4, 24, and 72 hours. MTT reagent was then added, and plates were incubated for an additional 24 hours before analysis. Error bars indicate standard deviation from the mean.