NOD HSCs exhibit enhanced migration to SDF-1 and adhesion to primary stroma. (A) Chemotaxis of NOR and NOD BMCs to SDF-1 gradient (200 ng/mL) compared with control without SDF-1. Data are expressed as percentage increase in migration compared with control (percentage migration without SDF-1 gradient). All experiments were repeated at least 2 times and were performed in quadruplicate (^*P < .0001). (B) Number of CFU-GM colonies formed by BMMNCs from NOR or NOD mice after migration to SDF-1 (^*P < .001). The number of colonies formed by cells migrating to SDF-1 gradient is shown as percent increase of colonies that were observed in cultures initiated with cells showing chemotaxis to control (no SDF-1) medium (arbitrary assumed to be 100%). (C) Adhesion assay: BM Sca-1⁺ cells from NOD (▪) or NOR mice (□) were allowed to adhere for 1 or 6 hours to primary BM stroma. Adherent cells were then placed in methylcellulose cultures and stimulated to grow CFU-GM colonies (mrIL-3 + mrGM-CSF) that were counted 7 days later. Data represent 2 experiments performed in triplicate (^*P = .005). (D) Adhesion molecule expression on NOD versus NOR KSL cells. Whole BM cells from NOD and NOR mice were stained for KSL cells and either adhesion molecule using mAbs against CD106, CD54, CD102, CD44, or CD62L FITC-labeled or the appropriate isotype control Abs. Data show the overlay of the different adhesion molecules (black) and the isotype control (gray). One representative experiment of 2 performed.