Effects of various inhibitors on the level of activation of downstream effector proteins in TEL-FGFR3 transfectants. (A) Western blotting using multiple antibodies for the phosphorylated forms of classical MAPK, p90 RSK, Akt, S6RP, and p38 MAPK in polyclonal TF-V5 transfectants (lane 1) and in cells transfected with TF-V5 that were incubated in the presence of 0.1% DMSO (lane 2), 25 μM SU5402 (lane 3), 25 μM U0126 (lane 4), 25 μM LY294 002 (lane 5), 25 μM wortmannin (lane 6), 2 μM rapamycin (lane 7), or 40 μM SB203580 (lane 8) for 6 hours. The eukaryotic initiation factor 4E (elF4E) was used for internal control. (B) Western blot analysis for STATs in polyclonal TF-V5 transfectants that were incubated in the absence of inhibitors (lane 1), and in cells transfected with TF-V5 that were incubated in the presence of 0.1% DMSO (lane 2), 25 μM SU5402 (lane 3), 25 μM U0126 (lane 4), 25 μM LY294002 (lane 5), 25 μM wortmannin (lane 6), 2 μM rapamycin (lane 7), or 10 μM SB203580 (lane 8). β-Actin was used for internal control.