Figure 6.
Figure 6. A high percentage of NKT cells among the liver mononuclear cells of the anti-CD3–conditioned recipients. The mononuclear cells from the liver of the anti-CD3– or TBI-conditioned NOD recipients 5 days after BMT were stained with anti–H-2q, anti-TCRαβ, anti-CD8, and CD1d-αGalCer-tetramer. The TCRαβ+ cells were first gated into H-2q+ and H-2q-, then both were shown in TCRαβ versus CD8. The gated TCRαβ+H-2q-CD8- cells were further shown in TCRαβ versus CD1d-αGalCer-tetramer. The percentage of each gated population among total cells was shown inside or beside the gating boxes. One representative of 4 measured recipients is shown. N/A indicates not available.

A high percentage of NKT cells among the liver mononuclear cells of the anti-CD3–conditioned recipients. The mononuclear cells from the liver of the anti-CD3– or TBI-conditioned NOD recipients 5 days after BMT were stained with anti–H-2q, anti-TCRαβ, anti-CD8, and CD1d-αGalCer-tetramer. The TCRαβ+ cells were first gated into H-2q+ and H-2q-, then both were shown in TCRαβ versus CD8. The gated TCRαβ+H-2q-CD8- cells were further shown in TCRαβ versus CD1d-αGalCer-tetramer. The percentage of each gated population among total cells was shown inside or beside the gating boxes. One representative of 4 measured recipients is shown. N/A indicates not available.

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