Figure 4.
Figure 4. Clonal deletion of donor- and host-reactive T cells in chimeric NOD recipients. Peripheral blood mononuclear cells (PBMNCs) from control FVB/N and NOD mice were stained with anti-TCRαβ versus anti-Vβ6, Vβ17, or Vβ10. The PBMNCs from long-term (> 24 weeks after BMT) chimeric recipients were stained with anti–H-2q, TCRαβ plus anti-Vβ6, Vβ17, or Vβ10. Donor-type (H-2q+) and host-type (H-2q-) TCRαβ+ cells were gated and then shown in TCRαβ versus Vβ6, Vβ17, or Vβ10. The percentage of H-2q+ and H-2q- TCRαβ+ cells among PBMNCs was shown in the gating boxes. The percentage of Vβ6+, Vβ17+, and Vβ10+ T-cell subsets among total T cells were shown above the gating boxes. One representative of 4 measured recipients is shown.

Clonal deletion of donor- and host-reactive T cells in chimeric NOD recipients. Peripheral blood mononuclear cells (PBMNCs) from control FVB/N and NOD mice were stained with anti-TCRαβ versus anti-Vβ6, Vβ17, or Vβ10. The PBMNCs from long-term (> 24 weeks after BMT) chimeric recipients were stained with anti–H-2q, TCRαβ plus anti-Vβ6, Vβ17, or Vβ10. Donor-type (H-2q+) and host-type (H-2q-) TCRαβ+ cells were gated and then shown in TCRαβ versus Vβ6, Vβ17, or Vβ10. The percentage of H-2q+ and H-2q- TCRαβ+ cells among PBMNCs was shown in the gating boxes. The percentage of Vβ6+, Vβ17+, and Vβ10+ T-cell subsets among total T cells were shown above the gating boxes. One representative of 4 measured recipients is shown.

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