Mixed-chimerism in anti-CD3 mAb preconditioned NOD recipients. (A) Flow cytometric analysis of donor-type (H-2^q+) cells including TCRαβ⁺, CD4⁺, CD8⁺, B220⁺, and Mac-1⁺/Gr-1⁺ cells in blood mononuclear cells of anti-CD3–conditioned recipients 10 weeks after BMT. The percentage of H-2^q+ donor-type and H-2^q- host-type cells are shown beside or in the gating boxes. One representative of 12 recipients is shown. (B) Stable multilineage chimerism in peripheral blood of NOD recipients for more than 28 weeks after BMT. ⬡ indicates CD4⁺; ▴, CD8⁺; , B220⁺; and ▾, Mac-1⁺/Gr-1⁺ cells. Values are mean ± SE of 12 recipients combined from 3 experiments (n = 12).