Phenotypic analysis of an AML. ML2 leukemia was analyzed for expression of representative markers by incubating cells with a specific mAb followed by PE-conjugated goat antimouse IgG antisera for single staining (A, gate on selected leukemic cells), while by FITC- or PE-conjugated isotype-specific goat antimouse second reagents for double staining (B, gate including both leukemic cells and normal lymphocytes). In panel A, white profiles represent isotype control.