Figure 4.
![Figure 4. Deficiency of both perforin and FasL causes decreased apoptosis of donor CD8+ T cells after BMT. (A) Wild-type (•) and cdd (▴) CD8+ T cells at 2 × 105/well were cultured with irradiated bm1 DCs (1 × 104/well) for 2 to 4 days. During the final 12 hours of culture, cells were pulsed with [3H] thymidine and assayed for proliferation. *P < .05 by Mann-Whitney U test. (B) Mice underwent transplantation as in Figure 1 and splenocytes were analyzed on day 10 after BMT by 2-color flow cytometry for expression of annexin and CD8+ cells. Gates were set for CD8+ cells, and the percentage of cells expressing annexin was determined. Data represent mean ± SD. wt versus cdd, *P < .05 by Mann-Whitney U test.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/105/5/10.1182_blood-2004-08-3036/6/zh80050574970004.jpeg?Expires=1724234134&Signature=cNgUCjBKg0ZnVJ3v-irbcsjufq2ao7RgU6m3bPNdw5cpCPGzWlq3qoDRw0lhwx6ZJjRXfX4BduN2w3dZFUcKZ9ctZFz2i-IWO5yszhSfuz1wjXee0TU5-NrbVARSZr2UTcMnmVNZPLo44b43X97SAKY-W-oo8~TXuIkN~m6IvLAwSVU5fZVJr3Avt4ba25tyTqlT~lCgsTTPAoZDxUjKDdkJPnY6cVQbnplG4~X31racc46AMbSi8l-w4JoNIbcXCejyplOWqJmwez-OFd0XBvL-j5DPEjPlwp9ODDVit3uOCFfnJXykz4uPHqLL3DQcQsYEgGOimkBJPVsmCNI-YQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Deficiency of both perforin and FasL causes decreased apoptosis of donor CD8+T cells after BMT. (A) Wild-type (•) and cdd (▴) CD8+ T cells at 2 × 105/well were cultured with irradiated bm1 DCs (1 × 104/well) for 2 to 4 days. During the final 12 hours of culture, cells were pulsed with [3H] thymidine and assayed for proliferation. *P < .05 by Mann-Whitney U test. (B) Mice underwent transplantation as in Figure 1 and splenocytes were analyzed on day 10 after BMT by 2-color flow cytometry for expression of annexin and CD8+ cells. Gates were set for CD8+ cells, and the percentage of cells expressing annexin was determined. Data represent mean ± SD. wt versus cdd, *P < .05 by Mann-Whitney U test.
