Effect of 17-AAG and/or LBH589 on pro-growth and pro-survival signaling proteins. Cotreatment with LBH589 and/or 17-AAG attenuates Bcr-Abl in primary CML-BC (B) and p-FLT-3 and FLT-3 in primary AML cells (A). Following treatment with 50 nM of LBH589 and/or 1000 nM 17-AAG for 24 hours, Western blot analyses of p-FLT-3, FLT-3, p-STAT5, STAT5, Pim-2, p-AKT, AKT, p-ERK1/2, and ERK 1/2 were performed on the cell lysates from sample no. 2. The levels of β-actin served as the loading control.