Figure 2.
Figure 2. “Cross-phenotyping” of IL-3Rα+ and IL-3Rα– BM progenitors for CD34 and FcγR expression. Lin–c-Kit+Sca-1–IL-7Rα– cells (black gate in panel B) were stained for CD34 versus FcγR expression and resolved (E) into CD34+FcγRlo (CMP), CD34+FcγRhi (GMP), and CD34–FcγRlo (MEP).6 For comparison, c-Kit+lin–Sca-1–IL-7Rα– cells were sorted into IL-3Rα– (A) and IL-3Rα+ (C) subsets, and then restained for CD34 and FcγR expression. c-Kit+IL-3Rα– cells include MEP and CMP phenotypes (D), and c-Kit+IL-3Rα+ cells include GMP and CMP phenotypes (F).

“Cross-phenotyping” of IL-3Rα+ and IL-3Rα BM progenitors for CD34 and FcγR expression. Linc-Kit+Sca-1IL-7Rα cells (black gate in panel B) were stained for CD34 versus FcγR expression and resolved (E) into CD34+FcγRlo (CMP), CD34+FcγRhi (GMP), and CD34FcγRlo (MEP). For comparison, c-Kit+linSca-1IL-7Rα cells were sorted into IL-3Rα (A) and IL-3Rα+ (C) subsets, and then restained for CD34 and FcγR expression. c-Kit+IL-3Rα cells include MEP and CMP phenotypes (D), and c-Kit+IL-3Rα+ cells include GMP and CMP phenotypes (F).

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