Figure 5.
Figure 5. FMLP and uPAR84-95 induce CD34+ KG1 and HSC migration by FPR activation. (A) KG1 cells, preincubated with buffer, 10-7 M fMLP, or 10-5 M fMLP, were plated in Boyden chambers and allowed to migrate toward 10-8 M fMLP or 10-6 M fMLP. (B) KG1 cells, preincubated with buffer, 10-7 M uPAR84-95, or 10-5 M fMLP, were plated in Boyden chambers and allowed to migrate toward 10-10 M uPAR84-95. Control migration toward 10-10 M scrambled uPAR84-95 (SC-uPAR84-95) was added. (C) Bone marrow CD34+ hematopoietic stem cells (BM HSC), preincubated with buffer or 10-5 M fMLP, were plated in Boyden chambers and allowed to migrate toward 10-10 M uPAR84-95. 100% values represent cell migration in the absence of chemoattractants. Values are the mean ± SD of 3 experiments performed in triplicate.

FMLP and uPAR84-95 induce CD34+ KG1 and HSC migration by FPR activation. (A) KG1 cells, preincubated with buffer, 10-7 M fMLP, or 10-5 M fMLP, were plated in Boyden chambers and allowed to migrate toward 10-8 M fMLP or 10-6 M fMLP. (B) KG1 cells, preincubated with buffer, 10-7 M uPAR84-95, or 10-5 M fMLP, were plated in Boyden chambers and allowed to migrate toward 10-10 M uPAR84-95. Control migration toward 10-10 M scrambled uPAR84-95 (SC-uPAR84-95) was added. (C) Bone marrow CD34+ hematopoietic stem cells (BM HSC), preincubated with buffer or 10-5 M fMLP, were plated in Boyden chambers and allowed to migrate toward 10-10 M uPAR84-95. 100% values represent cell migration in the absence of chemoattractants. Values are the mean ± SD of 3 experiments performed in triplicate.

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