Induction of a T-cell response to p276-290 by BBPS-bound β₂GPI in peripheral blood T cells from DR53⁺ healthy individuals in vitro. (A) Peripheral blood T cells from a representative DR53⁺ healthy individual were stimulated with DCs pulsed with BBPS-bound β₂GPI, BBPS, or β₂GPI alone, and the T-cell response to MalBP, GP-F, native β₂GPI, p276-290, or p306-320 was measured by IFN-γ production. A representative result from 2 independent experiments is shown. (B) T-cell response to p276-290 after priming with autologous DCs or macrophages pulsed with BBPS-bound β₂GPI, BBPS, or β₂GPI alone in 6 DR53⁺ healthy individuals. The p276-290–specific T-cell response was evaluated by IFN-γ production. The results from each of 6 individuals are indicated by a different symbol. Results are shown as mean and standard deviation.