Figure 5.
![Figure 5. Effect of GAS6 on the binding of [125I]VEGF-A165 at equilibrium to ECs. Cell monolayers were incubated for 90 minutes at room temperature in 0.2 mL binding buffer with 0.05 nM [125I] VEGF-A without (▪) or with the indicated concentrations of unlabeled VEGF-A165 (♦), and GAS6 (•). At the end of incubation, the cells were washed and solubilized with SDS 2%, and the radioactivity was counted. Data are the mean ± SD of 3 determinations in 1 experiment of 2 with similar results](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/105/5/10.1182_blood-2004-04-1469/6/zh80050574700005.jpeg?Expires=1724247767&Signature=XuRcU3~JddLwzMz7jNT4wA10u3ylziLap2wnHPNO5emNjgqoJoOO5tzG-T6exBfcJ4-UsmO61~cBAE3Cq~Onp7ONZJgEJlUjgpgUaOfEANAkHwEzstWtiXcQfDMUfWwO2ziB6FktZsjjqH0J4EvqZG7cX-K7qqqnk6L2YvUXbA0zEzC1TLR4G7A~hmpuq4DWHu2PDuTNRoFFS1vlqo2OzLU8kkjplHG3U2vQYnzrKztmL32-IlbjZGj3FFmUAhq44lz7rxPdiWOXnit7urTbWgtxLVMtAY~eS3fGiUWhUnP-iKOhCwpobRPjIdnNASTBB~8gzM2lAkzwYNJ0qJvw8Q__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Effect of GAS6 on the binding of [125I]VEGF-A165 at equilibrium to ECs. Cell monolayers were incubated for 90 minutes at room temperature in 0.2 mL binding buffer with 0.05 nM [125I] VEGF-A without (▪) or with the indicated concentrations of unlabeled VEGF-A165 (♦), and GAS6 (•). At the end of incubation, the cells were washed and solubilized with SDS 2%, and the radioactivity was counted. Data are the mean ± SD of 3 determinations in 1 experiment of 2 with similar results
