Inhibition of VEGFR-2 phosphorylation by GAS6. ECs were preincubated with GAS6 (1 ng/mL) for 20 minutes and consequently stimulated for 10 minutes with VEGF-A₁₆₅ (10 ng/mL). Cell lysates were immunoprecipitated with an anti–VEGFR-2 Ab, analyzed by SDS-PAGE, followed by immunoblotting with the indicated Abs. The results are representative of 4 similar experiments. (B) ECs were preincubated with GAS6 (1 ng/mL) for 20 minutes and consequently stimulated for 10 minutes with VEGF-A₁₆₅ (10 ng/mL). Cell lysates were immunoprecipitated with an anti–P-Tyr, analyzed by SDS-PAGE, followed by immunoblotting with the indicated Abs. The results are representative of 4 similar experiments.