GAS6 inhibits VEGF-A₁₆₅–mediated vascularization in CAM assay. CAMs were recorded at day 12 of incubation, 96 hours after the implant of a gelatin sponge soaked with VEGF-A₁₆₅ (50 ng) (A), GAS6 (50 ng) (B), VEGF-A₁₆₅ with GAS6 (20 ng) (C), or phosphate-buffered saline (E). Panel D shows the reverting effect of the presence Axl-Ig (200 ng) on the inhibitory effect of GAS6 (20 ng). Arrows in panels A and D indicate the new capillaries attracted by the sponges. Original magnification × 50. The picture is representative of 1 experiment of at least 4 performed. Images were processed by Image Proplus 4.0.