Absence of BCR-ABL p210 protein in 12B1 or 32Dp210 CRCL. 12B1 or 32Dp210 tumor lysates and CRCL from each of the lysates were prepared and separated on SDS-PAGE, followed by transfer to nitrocellulose for Western blotting. Presence of p210 BCR-ABL protein was probed using an antibody for c-ABL. A20 tumor lysates and CRCL served as negative controls.