Pim-1 is overexpressed in FLT3/ITD-positive samples from patients with AML, and its expression is decreased after treatment with CEP-701. (A) Samples from patients with AML were analyzed for Pim-1 expression by QPCR and compared with expression in normal bone marrow. The graph shows the relative expression levels of Pim-1 normalized to GAPDH in FLT3/ITD, FLT3/WT (wild-type) AML samples and normal bone marrow samples. The results show the mean and standard deviation for duplicate QPCR results from 3 independent experiments. The data were analyzed by Student t test and P values are shown. (B) Two FLT3/ITD-positive AML patient blast samples (patient 1 and patient 2) were incubated with 50 nM CEP-701 for 6 hours, and total RNA was harvested for QPCR. The results show the mean and standard deviation for triplicate QPCR results from 3 independent experiments.