Gene expression changes in EOL-1, MV4-11, and MOLM-14 cells treated with CEP-701. RNAs harvested from cell cultures treated as shown in Figure 1B were hybridized to cDNA microarray slides to examine changes in gene expression. RNA from uninhibited cells was labeled with Cy-5 dUTPs (green fluorescence signal). RNAs from inhibited cells or cells after inhibitor washout were labeled with Cy-3 dUTPs (red fluorescence signal). The uninhibited cell RNA was used as the control to examine changes in inhibited cell RNAs after 4 and 8 hours of inhibition or 4 hours and 8 hours release after inhibition. The inhibited RNA after 4 and 8 hours of inhibition or 4 hours and 8 hours of release after inhibition were represented as 4 hours inhibited, 8 hours inhibited, 4 hours released, and 8 hours released, respectively. The figure shows a pseudocolor representation of the intensity ratios of the raw data from the 2-channel fluorescent images. These data are quality filtered, and the top 15 genes ordered by degree of decreased expression in the EOL-1 cells inhibited by CEP-701 for 8 hours are shown.