Secretion of inhibitory cytokines by CTCL cells stimulated with apoptotic tumor cell-loaded DCs. (A) Supernatants were harvested from overnight cultures of CTCL cells (1 × 106/well) stimulated with DCs (1 × 105/well) that had been loaded with apoptotic tumor cells (1 × 106/well, CD3 treated), viable tumor cells (1 × 106/well, CD4 treated), γ-irradiated normal control CD4 T cells (1 × 106/well), or culture supernatant (none). Secreted IL-10 was detected by ELISA. Results are expressed as the mean ± SD of 3 cultures and are representative of 3 experiments. (B) Supernatants harvested from overnight cultures of CTCL cells (1 × 106/well) stimulated with DCs (1 × 105/well) that had been loaded with apoptotic tumor cells (1 × 106/well, CD3-treated), viable tumor cells (1 × 106/well, γ-1 isotype control treated), or culture supernatant (none). Secreted TGF-β was measured by ELISA. Results are expressed as the mean ± SD of 3 cultures and are representative of 3 experiments.
