Figure 7.
Figure 7. MoDCs activated with E coli and ATP secrete high levels of bioactive IL-23. (A) (left graph) Murine splenocytes were incubated with supernatant of unactivated or E coli plus ATP–activated MoDCs in the absence or presence of anti–IL-12p70 or anti–IL-12/23p40 mAb or an IL-23R/Fc fusion protein. (right graph) Murine splenocytes were incubated with different concentrations of recombinant human IL-23. Murine IL-17 production was measured by ELISA. (B) Human CD4+ T cells were sorted into naive and memory T-cell populations based on their expression of CD45RA and CD45RO, respectively, and were cultured in the presence of MoDC supernatants and CD3/CD28 T-cell expander beads. Isotype (IgG1), anti–IL-12p70, or anti–IL-12/23p40 mAbs were added as indicated. IL-17 was measured by ELISA. Data represent mean ± SEM of 5 separate experiments, n.d. indicates not done.

MoDCs activated with E coli and ATP secrete high levels of bioactive IL-23. (A) (left graph) Murine splenocytes were incubated with supernatant of unactivated or E coli plus ATP–activated MoDCs in the absence or presence of anti–IL-12p70 or anti–IL-12/23p40 mAb or an IL-23R/Fc fusion protein. (right graph) Murine splenocytes were incubated with different concentrations of recombinant human IL-23. Murine IL-17 production was measured by ELISA. (B) Human CD4+ T cells were sorted into naive and memory T-cell populations based on their expression of CD45RA and CD45RO, respectively, and were cultured in the presence of MoDC supernatants and CD3/CD28 T-cell expander beads. Isotype (IgG1), anti–IL-12p70, or anti–IL-12/23p40 mAbs were added as indicated. IL-17 was measured by ELISA. Data represent mean ± SEM of 5 separate experiments, n.d. indicates not done.

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