eNOS expression in the retina and eNOS modulation of retinal neovascularization. eNOS^–/– retinas were stained with NOS isoform-specific antibodies. In panels A-C, red depicts agglutinin staining for vessel visualization and green represents NOS antibody positive regions. Panel A is an eNOS^–/– retina stained with the eNOS-specific antibody. Panels B and C are eNOS^–/– stained with iNOS isoform-specific antibody. eNOS.gfp chimeric mice underwent the retinal ischemia model followed by perfusion with TRITC before eye enucleation and confocal imaging of the retinas. Panels D-G are red and green channel merged images from control (D,F) and treated (E,G) retinas. Original magnifications × 60 (D), × 4 (E), and × 10 (F). Panel G is a composite of images at original magnification × 60. Size bar is about 10 μM unless noted as about 100 μm.